07.07.17
US Patent No. 9,682,030 B2; AmorePacific Corp. Seoul, has patented a cosmetic composition comprising the polysaccharides of green tea as an effective ingredient, wherein the polysaccharides of green tea are characterized in that having a molecular weight of 100,000 to 300,000 daltons, with no having low molecular weight free proteins of below 30,000 Daltons and chlorophyll. The polysaccharides of green tea are prepared using the following steps:
a) removing chlorophyll and low molecular weight polyphenols from green tea powder using a solvent;
b) hot-water extracting water-soluble active ingredients from the green tea residue of step a) at a temperature of 30 to 40° C. for 6 to 8 hours; and
c) separating the polysaccharides of green tea removing low molecular weight free proteins from the hot-water extract of step b) by ultrafiltration and then separating the polysaccharides of green tea by ethanol precipitation wherein the polysaccharides of green tea have a molecular weight of 100,000 to 300,000 daltons.
a) removing chlorophyll and low molecular weight polyphenols from green tea powder using a solvent;
b) hot-water extracting water-soluble active ingredients from the green tea residue of step a) at a temperature of 30 to 40° C. for 6 to 8 hours; and
c) separating the polysaccharides of green tea removing low molecular weight free proteins from the hot-water extract of step b) by ultrafiltration and then separating the polysaccharides of green tea by ethanol precipitation wherein the polysaccharides of green tea have a molecular weight of 100,000 to 300,000 daltons.