Rising Bacterial Pathogen Shows Resistance to Quats in Common Cleaners: Research

Chemists at Emory University have published research that demonstrates multidrug-resistant Pseudomonas aeruginosa, a pathogen of particular concern in hospital settings, is showing a “surprising” level of resistance to cleaning agents. The Emory-lead research was published by The American Chemical Society Infectious Diseases.

The study also identifies biocides that are highly effective against P. aeruginosa, including a novel compound developed at Emory in collaboration with Villanova University.

The researchers describe how these biocides work differently than most disinfectants currently in use.

“We hope our findings can help guide hospitals to reconsider protocols for the sanitation of patient rooms and other facilities,” said William Wuest, Emory professor of chemistry and a senior author of the study. “We also hope that our findings of a new mechanism of action against these bacterial strains may help in the design of future disinfectant products.”

First authors of the study are Christian Sanchez (who did the work as an Emory PhD student in chemistry and, following graduation, joined the faculty at Samford University) and German Vargas-Cuebas, an Emory PhD candidate in microbiology.

“Resistance of pathogens to cleaning agents is an area that’s often overlooked, but it’s an important area of study, especially with the rise in antibiotic-resistant pathogens worldwide,” said Vargas-Cuebas.

Kevin Minbiole, professor of chemistry at Villanova, is a co-senior author of the paper.

“There are a handful of QACs [quaternary ammonium compounds] that have been the workhorse disinfectants for around 100 years, on the frontline of most homes and hospitals,” noted Wuest, whose lab is considered a leader in studies of QACs and other disinfecting agents. “Very little has been done to modify their structures because they have long worked so well against many common bacteria, viruses, molds and fungi and they’re so simple and cheap to make,” he added.

One issue Wuest and colleagues have identified is that some bacterial strains are developing resistance to QACs. That trend could cause serious problems for sanitation in hospitals.

A Pathogen of Priority

According to Centers for Disease Control and Prevention (CDC), multidrug-resistant P. aeruginosa is one of seven pathogens causing infections that increased in the US during the COVID-19 pandemic and remains above prepandemic levels.

Worldwide, P. aeruginosa causes more than 500,000 deaths annually and has been named a pathogen of critical priority by the World Health Organization.

P. aeruginosa is commonly found in the environment (including soil and fresh water). Reservoirs in hospital settings can include drains, taps, sinks and equipment washers. While the bacterium generally does not affect healthy people it can cause infections in individuals with cystic fibrosis and those who are immunocompromised. Patients with invasive devices such as catheters are also at risk due to the ability of P. aeruginosa to form biofilms on the surfaces of these devices, say experts.

P. aeruginosa, like other gram-negative bacteria, is enclosed in a second, fatty outer membrane that acts as a protective capsule, making it more difficult to kill, according to the scientists.

Testing & Results

The researchers tested 20 different drug-resistant strains of P. aeruginosa collected from hospitals around the world by the Walter Reed National Military Medical Center as part of the Multidrug-Resistant Organism Repository and Surveillance Network. The results showed that all 20 strains were at least partially resistant to QACs, also referred to as quats, and 80% of the strains were fully resistant to QACs.

“This mechanism has worked for 100 years essentially by slicing into the outer and inner membranes of a pathogen and destroying them,” Wuest says. “We were surprised to see the level at which that appears to no longer be the case.”

Improper use of cleaning agents may be one factor leading to resistance, Wuest theorizes.

“QACs don’t immediately kill,” he explained. “After application, it’s important to wait four or five minutes before wiping these cleaning agents away. It’s also important to use the right concentration. If used inappropriately, some bacteria can survive which can lead to them developing resistance.”

Greater use of cleaning agents during the covid-19 pandemic may have given P. aeruginosa and some other hard-to-kill pathogens more opportunities to develop resistance, he adds.

Testing Pathogen

For the current paper, the researchers also tested the resistance of the panel of multidrug-resistant P. aeruginosa strains against a new quaternary phosphonium compound, or QPC, developed in the Wuest and Minbiole labs. The results showed that the compound was highly effective at killing all 20 of the resistant P. aeruginosa strains.

“It works surprisingly well even at a low concentration,” said Vargas-Cuebas.

The researchers demonstrated that their novel QPC works not by piercing the protective outer capsule of a P. aeruginosa bacterium but by diffusing through this outer membrane and then selectively attacking the inner cellular membrane.

Wuest says it counterintuitive.

“You would think that the approach of conventional biocides, to take out both membranes, would be a more effective way to kill P. aeruginosa. Why does passively diffusing through the outer membrane and focusing on attacking the inner membrane make our QPC compound more effective? We don’t know yet. It’s like a magic trick,” he said.

They showed that this same mechanism underlies the effectiveness of two commercial antiseptics: octenidine, more commonly used in Europe as a hospital antiseptic, and chlorhexidine, a common ingredient in mouthwashes.

Wuest and his colleagues plan to continue research into how this newly identified mechanism may work against an array of pathogens and how that might translate into new biocides and more effective cleaning protocols in hospitals and other settings.

“Our work is paving the way for much-needed innovations in disinfectant research,” Wuest says.

Additional authors of the paper include Emory graduate student Marina Michaud, Emory undergraduate Shehreen Siddiqui and Emory PhD graduates Ryan Allen and Kelly Morrison-Lewis.

The work was funded by the National Institutes of Health.