New Research Digs Into the Complexity of Snail Mucus

Researchers examining the molecular composition of the mucus of a common garden snail say they have found a complex collection of proteins, including some identified as entirely novel.

In a newly published paper in Nature Communications, scientists profile the mucus of Cornu aspersum—the species used in beauty product formulation and often consumed as escargot—and detail the composition of three unique types of secretions: one that hydrates and protects its skin, another that works as a glue-like adhesive, and another that lubricates to allow the animal to move freely across surfaces.

“We were surprised that the mucus compositions were quite different, despite being produced by the same species,” said lead author Antonio Cerullo. Cerullo is a biochemistry Ph.D. student at the CUNY Graduate Center. “Even more so, the adhesive snail glue and the lubricious snail trail, which have completely opposite purposes, come from the same part of the snail. It was exciting to discover that very subtle differences in mucus compositions have huge impacts on their biological and material properties.”

The researchers say the hydrogels contain ions, sugars, and more than 70 proteins, including enzymes, mucins, lectins, and matrix proteins. About one-third of the proteins found in the mucus shared no similarity with any proteins in the global databases that were searched, said the researchers.

There are still many open questions about the macromolecular structure of mucus, say the researchers. Even human mucus, which has been studied much more extensively than snail mucus, is not well understood.

“Everyone is fascinated and disgusted by mucus. However, most people don’t realize just how complex and elegant these secretions are,” said the study’s principal investigator Adam Braunschweig, who is a faculty member with the Nanoscience Initiative at the Advanced Science Research Center at the CUNY Graduate Center (CUNY ASRC) and a professor of chemistry and biochemistry at the Graduate Center and Hunter College.

The data revealed subtle differences that accounted for vast differences in properties of the mucus. One example involves the use of calcium. Lubricating mucus has the least amount of calcium and the highest amount of calcium-binding proteins, while binding mucus has the opposite composition.

Snail mucus is currently being studied for applications in biomedicine, including surgical glues, as lubricants for eyes, joints, and medical implants, as well as drug delivery systems. More uses of snail mucus are being discovered every day, Braunschweig said.

Xi Chen, a CUNY ASRC researcher and professor at The City College of New York and the Graduate Center, and Mandë Holford, a chemistry professor at the CUNY Graduate Center and Hunter College were collaborators on the study.

The study was funded by the National Science Foundation, the Air Force Office of Scientific Research, and the US Space Force. Collaborators were supported by the Office of Naval Research, the Allen Institute, and the National Institutes of Health.