06.01.23
US Patent No. 11,661,568 B2 (Steven George Patterson, Jean-Pol Boutique, Johan Maurice Theo De Poortere, Nazarmohammad Gulamhussain); Procter & Gamble has been awarded a US patent for laundry detergent comprising a lipase and an ethylene oxide-propylene oxide-ethylene oxide (EO/PO/EO) triblock copolymer.
The copolymer comprises a first EO block, a second EO block, and a PO block. The first EO block and the second EO block are linked to the PO block.
The composition comprises between about 0.0001% and about 0.75%, by weight of lipase, based on active protein, wherein the lipase is selected from a lipase which is a polypeptide having an amino acid sequence which:
• has at least about 90% identity with the wild-type lipase derived from Humicola lanuginosa strain DSM 4109; or
• compared to said wild-type lipase, comprises a substitution of an electrically neutral or negatively charged amino acid at the surface of the three-dimensional structure within 15 angstroms of E1 or Q249 with a positively charged amino acid; or
• a mixture of (a) and (b).
The copolymer comprises a first EO block, a second EO block, and a PO block. The first EO block and the second EO block are linked to the PO block.
The composition comprises between about 0.0001% and about 0.75%, by weight of lipase, based on active protein, wherein the lipase is selected from a lipase which is a polypeptide having an amino acid sequence which:
• has at least about 90% identity with the wild-type lipase derived from Humicola lanuginosa strain DSM 4109; or
• compared to said wild-type lipase, comprises a substitution of an electrically neutral or negatively charged amino acid at the surface of the three-dimensional structure within 15 angstroms of E1 or Q249 with a positively charged amino acid; or
• a mixture of (a) and (b).